Plasmid pMKE2

Description:

Plasmid pMKE2 is a bifunctional E. coli-thermus sp. Vector, under the control of the Pnar promoter, with multiple cloning sites that allow for the direct cloning of genes to be expressed in T. thermophilus. The Pnar promoter, activated by nitrate and anoxia, controls the expression of the nitrate reductase operon (nar operon), a key element for growth under anaerobic conditions.

The ability to grow under anaerobic conditions is encoded within the portable element, nar operon, which can be transferred by cloning in pMKE2. Overexpression of thermozymes is specifically induced through the combination of nitrate and anoxia.
The pMKE2 contains a modified Pnar promoter which allows the production of active enzymes in larger amounts than those obtained using E. coli as cell factory (1).

1. Moreno R. et al. (2005). Appl Environ Microbiol 71: 591-3.

Contents:

1 µg Plasmid pMK18 (100 ng/µl) is supplied in ultrapure water.