Plasmid pMKE1

Description:

Plasmid pMKE1 is a bifunctional E. coli-thermus sp. Vector, under the control of Pnar promoter, with multiple cloning sites that allow for the direct cloning of genes expressed in T. thermophilus. The Pnar promoter, activated by nitrate and anoxia, controls the expression of the nitrate reductase operon (nar operon), a key element for growth under anaerobic conditions.

The ability to grow under anaerobic conditions is encoded within the portable element, nar operon, which can be transferred by cloning in pMKE1. Overexpression of thermozymes is specifically induced through the combination of nitrate and anoxia (1).

1. Moreno R. et al. (2003). Plasmid 49: 2-8.  

Contents:

1 µg Plasmid pMK18 (100 ng/µl) is supplied in ultrapure water.