PRODUCTS >> LABS BIOTOOLS >> Reverse Transcription  
 
 


REVERSE TRANSCRIPTION

Product

Manual

Biotools High Retrotrasncriptase - Starter Kit
High RNase Inhibitor

Biotools High Retrotrasncriptase

REVERSE TRANSCRIPTION PCR

Product

Detection Method

One Step

Two Step

GC-rich

Manual

RetroTools® TwoStep kit

End-point

NO

YES

+

Std.

Free

High ScripTools OneStep kit

End-point

YES

NO

++

High ScripTools Quantimix Easy Kit

Real Time

YES

NO

++

High ScripTools Quantimix Easy Probes Kit

Real Time

YES

NO

++

Reverse Transcription PCR (RT-PCR) can be performed as a One-Step or Two-Step reactions. Two-Step RT-PCR is useful for detecting multiple messages from a single RNA sample or when you need maximum flexibility in selecting PCR conditions.

The One-Step RT-PCR method offers the capability of performing both cDNA synthesis and PCR amplification in a single tube which prevents contamination and promotes rapid processing for a large number of samples. Since the entire cDNA is amplified, One-Step RT-PCR can also provide greater sensitivity.

Biotools offers RT-PCR kits with high transcriptional efficiency, as well as high yield, sensitivity and specificity for customer RT-PCR needs, including end-point and real time quantitative RT-PCR detection kits.

Almost all Biotools RT-PCR kits have been developed with a new thermostable retrotranscriptase that offers advantages with respect to other enzymes available elsewhere.

  • Robust cDNA synthesis across a broad range of temperatures from 37 to 77 °C
  • Excellent cDNA yields
  • Ability to reverse transcribe through a secondary structure
  • High stability

 

Related Products

  1. dNTPs
  2. Primers and Probes
  3. Fluorophores and Dyes
  4. PCR Enhancer
  5. Amplification Controls
  6. Buffers and Solutions
  7. DNA and RNA Purification
  8. Electrophoresis Reagents

 

   
 



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