REVERSE TRANSCRIPTION PCR
Reverse Transcription PCR (RT-PCR) can be performed as a One-Step or Two-Step reactions. Two-Step RT-PCR is useful for detecting multiple messages from a single RNA sample or when you need maximum flexibility in selecting PCR conditions.
The One-Step RT-PCR method offers the capability of performing both cDNA synthesis and PCR amplification in a single tube which prevents contamination and promotes rapid processing for a large number of samples. Since the entire cDNA is amplified, One-Step RT-PCR can also provide greater sensitivity.
Biotools offers RT-PCR kits with high transcriptional efficiency, as well as high yield, sensitivity and specificity for customer RT-PCR needs, including end-point and real time quantitative RT-PCR detection kits.
Almost all Biotools RT-PCR kits have been developed with a new thermostable retrotranscriptase that offers advantages with respect to other enzymes available elsewhere.
- Robust cDNA synthesis across a broad range of temperatures from 37 to 77 °C
- Excellent cDNA yields
- Ability to reverse transcribe through a secondary structure
- High stability
- Primers and Probes
- Fluorophores and Dyes
- PCR Enhancer
- Amplification Controls
- Buffers and Solutions
- DNA and RNA Purification
- Electrophoresis Reagents