RETROTOOLS® TWOSTEP KIT
RetroTools® TwoStep kit
with Standard Reaction Buffer
Description:
The RetroTools® TwoStep kit is a TwoStep RT-PCR method which includes a recombinant thermostable Tth cDNA/DNA Polymerase. The Tth enzyme exhibits both DNA polymerase and retro-transcriptase activities.
The ability of the Tth enzyme to reverse transcribe at elevated temperatures minimises the problems encountered with strong secondary structures in RNA. Higher reaction temperatures also result in increased specificity of primer hybridization and extension.
The kit is applicable, but not limited to, the following analyses:
- Amplification of RNAs containing GC-rich regions
- Amplification of RNAs with high degree of secondary structure
Contents:
RetroTools® TwoStep kit is provided with RetroTools Polymerase (Tth cDNA/DNA Polymerase), RT Buffer, MnCl2, dNTPs, and Standard Reaction Buffer with MgCl2, in a separate tube.
RetroTools® TwoStep kit
with Reaction Buffer MgCl2 Free
Description:
The RetroTools® TwoStep kit is a TwoStep RT-PCR method which includes a recombinant thermostable Tth cDNA/DNA Polymerase. The Tth enzyme exhibits both DNA polymerase and retro-transcriptase activity.
The ability of the Tth enzyme to reverse transcribe at elevated temperatures minimises the problems encountered with strong secondary structures in RNA. Higher reaction temperatures also result in increased specificity of primer hybridization and extension.
The kit is applicable, but not limited to, the following analyses:
- Amplification of RNAs containing GC-rich regions
- Amplification of RNAs with high degree of secondary structure
Contents:
RetroTools® TwoStep kit is provided with RetroTools® Polymerase (Tth cDNA/DNA Polymerase), RT Buffer, MnCl2, dNTPs, Reaction Buffer MgCl2 Free, and 50 mM MgCl2, in a separate tube.
Product |
Cat N° |
Format |
RetroTools® Two-Step kit DNA Amplification Buffer with MgCl2 |
10.053 |
100 rxn |
RetroTools® TwoStep kit DNA Amplification Buffer MgCl2 FREE +50 mM MgCl2 |
10.059 |
100 rxn |
|
