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Description:
BIOTYPAP kit is a qualitative analysis to detect human papillomavirus (HPV) by DNA amplification. Furthermore, the kit differentiates between the principal oncogenic by restriction digest (RFLP). The test detects 32 varieties of HPV in cervix samples and it differentiates between generic and oncogenic HPV genotypes.
Viral DNA present in positive samples is amplified by using specific primers, which hybridise in homologous sequences into viral genome. Amplification products are digested using restriction endonucleases rendering specific patterns for the principal oncogenic genotypes.
The assay consists of a multiplex amplification reaction, with two pair of primers. One of them hybridises with sequences common to all tested genotypes to detect HPV presence; the second pair of primers hybridises with a specific sequence for oncogenic varieties of virus.
Contents:
BIOTYPAP kit is supplied with:
- HPV Master mix: The mix includes all the amplification reagents for the HPVs detection and differentiation of generic and oncogenic genotypes, except MgCl2 and DNA polymerase
- MgCl2
- DNA Polymerase
- Mixes A; B; C; D and E: Restriction enzymes A to E to perform the typing
- Buffers 1; 2; 3; 4 and 5: Reaction buffers for enzymes A to E, respectively
- Positive control: Non-infective DNA sequences of both, generic and oncogenic HPV
Product |
Cat N° |
Format |
BIOTYPAP kit |
90.017 |
96 rxn |
* Kit available upon request, please check delivery time |
