SpeedTools Plasmid DNA Purification kit

Description:

The SpeedTools Plasmid DNA Purification kit is designed for a rapid and easy preparation of up to 20 µg of highly pure plasmid DNA from 0.5 to 2 ml bacterial suspension (mini preps). It is based on a modified alkaline lysis procedure followed by binding of the plasmid DNA to a silica membrane column in a comfortable spin format.

Starting material:

fresh cultured bacterial suspension                    frozen bacteria pellets

The obtained plasmid DNA is ready to use for a wide variety of different downstream applications: PCR, reaction enzyme digestion, labeling, cloning, in vitro transcription, and capillary sequencing.

Advantages:

• Easy method: just binding, washing and eluting
• Very fast procedure (less than 10 min)
• Reproducible recovery rates of highly purified pDNA
• Column method, no time- consuming ethanol precipitation steps
• Ultra pure, ready to use pDNA 

Contents:

SpeedTools Plasmid DNA Purification kit is provided with Resuspension Buffer, Lysis Buffer, Neutralisation Buffer, Washing Buffer, Binding Columns, Collecting tubes and Elution Buffer.