PolymerSlide™G

Description:

The PolymerSlide™G is specially designed for covalent attachment of proteins, polypeptides and peptides. The slide surface is coated with a three dimensional polymer layer with a functionally modified aldehyde. Amino groups bind to the reactive aldehyde of the polymer through a covalent interaction. Polymer chains provide optimal conditions for the preservation of the physiological status of adsorbed-proteins (e.g. activity).

Protein modification or additional steps are not required for spotting. To get the best printing of proteins we recommend the use of Protein Spotting Buffer A (cat N° 71.111 and 71.112).
Recommended uses:

• Protein microarrays (wide applications)
• Peptide microarrays (wide applications)

Contents:

The PolymerSlides™G are provided in different formats: 25, 100 and 500 slides.

SLIDE SURFACES

One of the major limitations of microarray analysis is the quality of coating chemistries on the glass surface. Deficient quality arrays printed on coated glass slides lead to a loss of sensitivity and variations in spot size, shape, and even probe content.
CapitalBio offers a full set of accurately modified glass slides suitable for both contact and non-contact printing with various nucleic acids and proteins.

CapitalBio has also developed various cutting-edge biochips utilising microfluidic technology, microprocessor technology and bio-electro-magnetic technology. Slides are coated with several layers of nitrocellulose, nylon, polilysine and agarose/ achrylamide, as described in different patents. Different functional groups can be used inside this microfluidic matrix.

We have studied and optimised a range of reliable and reproducible glass slides suitable for different microarrays. The following table has been designed for you to select the appropriate slide for each microarray application.

All slides commercialised by Biotools are manufactured under stringent conditions, including: contact angle measurement to monitor surface wetting characteristics, and background scanning for coating homogeneity.