OPEpoxySlide^TM

Description:

The OPEpoxySlide^TM has been designed for a wide range of microarray platforms (proteins, amino-modified molecules, oligonucleotides, and PCR products). The epoxy bound surface is one of the most robust and efficient ones for microarrays. Amino, hydroxi and mercapto groups from proteins bind to the highly active epoxy groups on the coated slide surface through covalent interactions. On the other hand, amine groups from PCR products, oligonucleotides or amino-modified molecules react with the slide’s epoxy groups, through a strong union.

Amines present in spotting buffers may compete with probes for the attachment to epoxy groups. For printing samples on OPEpoxySlides^TM we recommend the use of spotting buffers that are free of amines (e.g. Protein Spotting Buffer B: cat N° 71.114 and 71.115).

Recommended uses:

• Transcriptional profiling
• Protein microarrays (wide applications)
• Oligonucleotide microarrays (wide applications)

Contents:

The OPEpoxySlides™ are provided in different formats: 25, 100 and 500 slides.

SLIDE SURFACES

One of the major limitations of microarray analysis is the quality of coating chemistries on the glass surface. Deficient quality arrays printed on coated glass slides lead to a loss of sensitivity and variations in spot size, shape, and even probe content.
CapitalBio offers a full set of accurately modified glass slides suitable for both contact and non-contact printing with various nucleic acids and proteins.

CapitalBio has also developed various cutting-edge biochips utilising microfluidic technology, microprocessor technology and bio-electro-magnetic technology. Slides are coated with several layers of nitrocellulose, nylon, polilysine and agarose/ achrylamide, as described in different patents. Different functional groups can be used inside this microfluidic matrix.

We have studied and optimised a range of reliable and reproducible glass slides suitable for different microarrays. The following table has been designed for you to select the appropriate slide for each microarray application.

All slides commercialised by Biotools are manufactured under stringent conditions, including: contact angle measurement to monitor surface wetting characteristics, and background scanning for coating homogeneity.