Certamp kit for Long Amplifications

Description:

Certamp kits include an exclusive combination of proof-reading and non-proofreading DNA Polymerases, together with cofactors and an enhancing buffer to increase the overall yield of long and/or difficult amplification reactions.

The enzyme mix maintains maximum efficiency while keeping the lowest error rate, as the low processivity of proofreading enzymes is compensated by the high processivity of non-proofreading enzymes.

Certamp kit for Long Amplifications is suitable for:

• Amplification of DNA fragments of up to 40 kb from λ fage or similar DNAs
• Amplification of DNA fragments of up to 15 kb from genomic DNA

Certamp kit for Long Amplifications amplifies long DNA fragments from λ fage Fragments of 5 Kb; 13 Kb and 20 Kb were amplified from λ DNA with high yield. Lines 1; 3 and 5: Negative Controls. M1: 1 Kb Ladder; M2: λ /Hind III.

Contents:

Certamp kit for Long Amplifications is provided with Certamp Enzyme Mix, two buffers (10X Reaction Buffer MgCl2 Free and 10X Certamp Long Buffer MgCl2 Free)and 50 mM MgCl₂.