Biotools Pfu DNA Polymerase

Description:

High-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3’-5’ exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).

Biotools Pfu DNA Polymerase generates blunt-ended amplification products, enabling their cloning into blunt-ended vectors. It is suitable for applications including:

• Cloning
• Mutagenesis
• Achieving greater fidelity than with Taq DNA polymerase
• Amplifying fragments of up to 5 kb

Contents:

Biotools Pfu DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer including 2 mM MgCl₂ or a reaction buffer without MgCl₂ (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl₂ for optimising the magnesium concentration.