Biotools HotSplit DNA Polymerase

Description:

Biotools HotSplit DNA Polymerase is ideal for “hot start” amplification of DNA fragments with improved specificity over our conventional DNA Polymerase. It uses thermolabil blocking groups that act over the amino-acid residues involved in enzyme polymerization. During the PCR initial denaturation step, the blocking groups are released and the active DNA Polymerase is available into the reaction.

The enzyme improves PCR specificity by preventing the extension of non-specifically bound primers during the reaction setup and the first heating cycle. Thus, it prevents the formation of primer-dimers.

Biotools Hot Split DNA Polymerase is ideal when using robotic PCR systems that include incubations at room temperature prior to temperature cycling. This enzyme has applications in:

• Improved specificity and yields of PCR compared to conventional DNA Polymerase
• Amplification of PCR products of up to 5 kb
• Reduced error rates (1 - 10 x 10-⁶)

Advantages:

• Prevents the formation of primer-dimers
• Reduces non-specific amplification and primer degradation
• The enzyme is inactive at room temperature, and the activity is recovered by a 5-min  incubation step at 95 °C
• Increased specificity

Conventional DNA Polymerase	BioTools Hot Split DNA Polymerase
Hot Split DNA Polymerase eliminates primer-dimers Fragments of different species of Plasmodium were amplified from human gDNA in a multiplex reaction (lines 1 to 4 and 6 to 9). Lines 5 and 10: Negative Controls. M: Molecular Size Marker.

Contents:

Biotools HotSplit DNA Polymerase (1U/µl) is supplied with a 10 X Reaction Buffer MgCl₂ Free plus a separate tube of MgCl₂ for optimising the magnesium concentration.