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Biotools DNA Polymerase Gel Form

Biotools has developed a new PCR product using one of its international patents: GELIFICATION. the process is simple and in less than 10 minutes you have your 96 PCR/ qPCR reaction runing:

 

 
 

STEP 1:

Receive your plates

 

STEP 2: 5 sec.

Remove the film from the plate

STEP 3: 5 min.

Pipette your samples

STEP 4: 30 sec.

Seal one of the films

STEP 5: 1 min.

Insert the plate in the Thermal Cycler

 

Features:

 

READY-TO-USE
One well- One reaction format
Ready for in-field manipulation
No optimization needed

LESS MANIPULATION & LESS CONTAMINATION *
Only need to add primers and DNA sample
*Customized plates available: give us your primers and we will include them in the Gelified reaction

SHIPMENT AT ROOM TEMPERATURE AND STORAGE AT 4ºC
Stable for long time periods at 4ºC

HOT-START EFFECT
Reaction starts when the Gel is completely rehydrated with the increase of temperature

NO NEED TO RESUSPEND
Reaction will become liquid automatically with the increase of temperature (no handling)

ADVANTAGES vs LYOPHILIZATION
Components keep their activity
No need to resuspend
Final concentration is mantained

 

 

The Biotools DNA Polymerase Gel Form and the gelified mix reagents can be stored at 4 °C respectively for long periods of time. The Ready-to-Use format minimises risk of contaminations, while promoting rapid sample processing ideal for routine applications.

This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5’-3’ polymerase and 5’-3’ exonuclease activities, and reduced error rates (1 - 10x 10-6). Thermostable enzyme perfect for routine amplifications including:

  • Amplification of PCR products of up to 5 Kb
  • Colony screening
  • Recombinant cDNA construction
  • Genotyping
  • Routine DNA amplifications

Biotools DNA Polymerase Gel Form is available in Vials (2 U/vial) and in 96 well Plates (2 U/well).

High yield of PCR reactions with Biotools DNA Polymerase Gel Form

A fragment of 260 bp of DNA was amplified using Biotools DNA Polymerase Gel Form (lanes 2 and 3) and Biotools DNA Polymerase (lanes 5 and 6). Lines 1 and 4: Negative control.

Hot Start effect of Biotools DNA Polymerase Gel Form

A fragment of 260 bp of DNA was amplified using Biotools DNA Polymerase
Gel Form (lane 2); a commercial Hot Start DNA Polymerase (lane 1) and Conventional DNA Polymerase (lane 3).
Lane 4: Negative control.
M: Molecular Size Marker.

Contents:

Biotools DNA Polymerase Gel Form 96 well Plates is supplied with 10 X Standard Reaction Buffer including 2 mM MgC2 and dNTPs.

Biotools DNA Polymerase Gel Form Vials(0,2 vials) 96 vials. Each vial includes Standard Reaction Buffer with 2 mM MgCl2, dNTPs, and DNA polymerase..

 

Product

Cat N°

Format

Biotools DNA Polymerase Gel Format p96 Plates (2U/well)

10.542

10 plates

Biotools DNA Polymerase Gel Format p96 Plates (2U/well)

10.543

20 plates

Biotools DNA Polymerase Gel Form  Vials –12x8 strip vials (2U/vial)

10.151

96 vials

 
   
 



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