The Biotools DNA Polymerase Gel Form and the gelified mix reagents can be stored at 4 °C respectively for long periods of time. The Ready-to-Use format minimises risk of contaminations, while promoting rapid sample processing ideal for routine applications.
This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5’-3’ polymerase and 5’-3’ exonuclease activities, and reduced error rates (1 - 10x 10-6). Thermostable enzyme perfect for routine amplifications including:
- Amplification of PCR products of up to 5 Kb
- Colony screening
- Recombinant cDNA construction
- Genotyping
- Routine DNA amplifications
Biotools DNA Polymerase Gel Form is available in Vials (2 U/vial) and in 96 well Plates (2 U/well).
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High yield of PCR reactions with Biotools DNA Polymerase Gel Form
A fragment of 260 bp of DNA was amplified using Biotools DNA Polymerase Gel Form (lanes 2 and 3) and Biotools DNA Polymerase (lanes 5 and 6). Lines 1 and 4: Negative control.
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Hot Start effect of Biotools DNA Polymerase Gel Form
A fragment of 260 bp of DNA was amplified using Biotools DNA Polymerase
Gel Form (lane 2); a commercial Hot Start DNA Polymerase (lane 1) and Conventional DNA Polymerase (lane 3).
Lane 4: Negative control.
M: Molecular Size Marker.
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Contents:
Biotools DNA Polymerase Gel Form 96 well Plates is supplied with 10 X Standard Reaction Buffer including 2 mM MgC2 and dNTPs.
Biotools DNA Polymerase Gel Form Vials(0,2 vials) 96 vials. Each vial includes Standard Reaction Buffer with 2 mM MgCl2, dNTPs, and DNA polymerase..
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